Table 1.
Phenotypic characteristics of 8 reference strains and 41 clinical isolates originally identified as either C. guilliermondii or C. famataa
| Strain (accession no.) | Original species identification | Growth at 37/40/42°Cb | Formation of pseudohyphae on CMAc | Color on CHROMagar | ATB strip (ID32C) profile | Species identified by ITS sequencing |
|---|---|---|---|---|---|---|
| CBS 7418 | C. palmioleophila | +/+/− | B | Turquoise | 5367352315E− | C. palmioleophila |
| W38018-09 | C. famata | +/+/− | B | Turquoise/rose | 5367352315E− | C. palmioleophila |
| W27955-07 | C. guilliermondii | +/+/− | B | Turquoise/rose | 5367352315E− | C. palmioleophila |
| T47982-09 | C. famata | +/+/− | B | Turquoise/rose | 5367352315E− | C. palmioleophila |
| M45383-09 | C. famata | +/+/− | B | Turquoise/rose | 5367352315E− | C. palmioleophila |
| W29590-09 | C. famata | +/+/− | B | Turquoise/rose | 5367352315E− | C. palmioleophila |
| M62963-09 | C. guilliermondii | +/+/− | B | Turquoise/rose | 5367352315E− | C. palmioleophila |
| M67303-09 | C. famata | +/+/− | B | Turquoise/rose | 367352315E−d | C. palmioleophila |
| F27811-07 | C. guilliermondii | +/+/− | − | Turquoise/rose | 5367352215E−d | C. palmioleophila |
| UKNEQAS 4447 | C. guilliermondii | +/+/− | − | Dark purple | 7577755715E+ | C. guilliermondii |
| UKNEQAS 8112 | C. guilliermondii | +/+/− | − | Light purple | 7577752517E+ | C. guilliermondii |
| CBS 6021 | C. guilliermondii | +/+/− | + | Purple | 7577377315E+ | C. guilliermondii |
| A3970-87 | C. guilliermondii | +/+/− | − | Purple | 5577752717E+ | C. guilliermondii |
| F15415-06 | C. guilliermondii | +/+/− | + | Dark purple | 7177352117E− | C. guilliermondii |
| W63031-08 | C. guilliermondii | +/+/− | − | Purple | 7577352117E+ | C. guilliermondii |
| T51653-07 | C. famata | +/+/− | − | Light purple | 7177352117E− | C. guilliermondii |
| F38277-07 | C. guilliermondii | +/+/− | − | Light purple | 7577352117E+ | C. guilliermondii |
| A173-94 | C. guilliermondii | +/+/w | + | Light purple | 7577352117E+ | C. guilliermondii |
| A336-99 | C. guilliermondii | +/+/− | − | Light purple | 7577357317E+ | C. guilliermondii |
| A331-75 | C. guilliermondii | +/+/w | + | Light purple | 7577752113E+ | C. guilliermondii |
| A194-53 | C. guilliermondii | +/+/w | − | Purple | 7577752117E+ | C. guilliermondii |
| A2189-86 | C. guilliermondii | +/+/− | − | Purple | 7577350515E+ | C. guilliermondii |
| A2551-86 | C. guilliermondii | +/+/− | − | Purple | 5577350117E+ | C. guilliermondii |
| W56260-07 | C. guilliermondii | +/+/− | − | Light purple | 7177352117E− | C. guilliermondii |
| A221-73 | C. guilliermondii | +/+/− | − | Purple | 7577352117E+ | C. guilliermondii |
| PF6934-86 | C. guilliermondii | +/+/− | − | Purple | 7577352117E+ | C. guilliermondii |
| A149-49 | C. guilliermondii | +/+/− | − | Purple | 7577750115E+ | C. guilliermondii |
| A2745-86 | C. guilliermondii | +/+/− | − | Purple | 7577752115E+ | C. guilliermondii |
| F44185-06 | C. guilliermondii | +/+/− | − | Purple | 7577752117E+ | C. guilliermondii |
| A1499-76 | C. guilliermondii | +/+/− | + | Purple | 7577752317E+ | C. guilliermondii |
| A1469-70 | C. guilliermondii | +/+/− | + | Purple | 7577750315E+ | C. guilliermondii |
| M8464-08 | C. guilliermondii | +/+/− | − | Light purple | 7577750315E+ | C. guilliermondii |
| H31147-09 | C. guilliermondii | +/+/− | − | Purple | 7577352117E+ | C. guilliermondii |
| T38215-06 | C. guilliermondii | +/+/− | − | Purple | 7577350117E− | C. guilliermondii |
| CBS 9966 | C. fermentati | +/+/− | − | Purple | 5577350115E+ | C. fermentati |
| F49572-08 | C. guilliermondii | +/+/− | − | Purple | 7577350117E+ | C. fermentati |
| M10611-04 | C. guilliermondii | +/+/− | − | Purple | 7577350117E+ | C. fermentati |
| W63245-01 | C. guilliermondii | +/+/− | + | Purple | 7577750717E+ | C. fermentati |
| T38768-04 | C. guilliermondii | +/+/w | − | Purple | 7577350117E+ | C. fermentati |
| T32779-07 | C. guilliermondii | +/+/w | − | Purple | 7577750117E+ | C. fermentati |
| M39632-09 | C. guilliermondii | +/+/w | − | Dark purple | 7577752317E− | C. fermentati |
| UKNEQAS 4620 | C. lusitaniae | +/+/+ | − | Purple | 5157370317E+ | C. lusitaniae |
| H45593-09 | C. famata | +/+/+ | + | Purple | 5377370317E+ | C. lusitaniae |
| H27507-04 | C. famata | +/+/+ | − | Light red | 5777770317E+ | C. lusitaniae |
| F47819-04 | C. famata | +/+/+ | − | Light red | 5757770317E+ | C. lusitaniae |
| CBS 572 | C. intermedia | −/−/− | + | Purple | 5377340733E+ | C. intermedia |
| A933-78 | C. famata | −/−/− | + | Light purple | 5177360137E+ | C. intermedia |
| F61108-07 | C. famata | −/−/− | + | Dark purple | 5177360137E+ | C. intermedia |
| CBS 796 | C. famata | −/−/− | − | Light red | 5577755117E+ | C. famata |
a
Growth at 37°C, 40°C, and 42°C, the presence of pseudohyphae on corn meal agar, and the colony color on CHROMagar were analyzed. All isolates were analyzed biochemically by 2nd day ID32C reads, and the ITS1/ITS2 region was sequenced.
b
w, weak growth.
c
B, budding present, but no pseudohyphae present.
d
Unacceptable ATB strip profile. Digits in gray differ from the consensus C. palmioleophila ID32C profile.