Table 2.
Genes studiedc
| Gene(s) | Size of analyzed fragment (bp) | %G+C | No. of polymorphic sites | No. of alleles | dN/dS | Maximum % of nt divergencea | % of nt divergence between type strain sequencesb |
||
|---|---|---|---|---|---|---|---|---|---|
| MabsT versus MmasT | MabsT versus MbolT | MbolT versus MmasT | |||||||
| argH | 480 | 66.8 | 40 | 19 | 0.1529 | 5.47 | 4.58 | 3.54 | 2.92 |
| cya | 510 | 68.2 | 31 | 25 | 0.0029 | 3.62 | 1.96 | 1.96 | 2.75 |
| glpK | 534 | 62.7 | 25 | 16 | 0.0380 | 2.48 | 1.69 | 1.31 | 0.94 |
| gnd | 480 | 65.2 | 33 | 20 | 0.0307 | 4.73 | 2.29 | 4.58 | 3.54 |
| murC | 537 | 68.6 | 39 | 16 | 0.1259 | 4.44 | 2.05 | 3.54 | 3.35 |
| pgm | 495 | 62.2 | 29 | 25 | 0.1900 | 2.02 | 1.41 | 1.41 | 0.81 |
| pta | 486 | 66.1 | 20 | 20 | 0.0110 | 2.74 | 1.85 | 1.65 | 2.26 |
| purH | 549 | 65.6 | 31 | 21 | 0.0502 | 2.98 | 1.82 | 2.00 | 0.18 |
| All MLSA genes | 4,071 | 65.7 | 248 | 79 | ND | 2.85 | 2.19 | 2.43 | 2.01 |
| rpoB | 752 | 65.6 | 43 | 22 | 0.0827 | 4.79 | 3.32 | 4.12 | 1.46 |
a
Maximum nucleotide divergence found between the 123 strains studied.
b
M. abscessus CIP 104536T, M. massiliense CIP 108297T, and M. bolletii CIP 108541T.
c
ND, not done; dN/dS, ratio of the rate of nonsynonymous substitutions (dN) to the rate of synonymous substitutions (dS); MabsT, M. abscessus type strain sequence; MbolT, M. bolletii type strain sequence; MmasT, M. massiliense type strain sequence.