Figure 3.
Analysis of caspase-4 enzyme activity in HEK and JEG-3 cells. The fluorochrome-labelled target peptide for caspase-4 was applied to JEG-3 and HEK cells. After intracellular peptide cleavage, the free fluorochrome, which is a measure of the enzyme activity, was analysed by HPLC. The figure shows the arithmetic mean of 3 independent experiments and the calculated standard error.