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. Author manuscript; available in PMC: 2011 Feb 23.
Published in final edited form as: J Med Genet. 2010 Aug 15;47(11):745–751. doi: 10.1136/jmg.2009.076703

Figure 3.

Figure 3

Testing for the 4qA161 haplotype by sequencing through SNP3 and SNP6 on the reverse strand. Sequence alignment of the 4qA alleles and 10qA166 allele illustrates the 4q-specific nature of PCR primer Bts-R. The BtsI site overlapping the primer in 10qA166 is highlighted (A). PCR with Bts-R as one of the primers followed by sequencing the reverse strand allows identification of the 4qA161haplotype. As described in the text, digestion with BtsI to eliminate 10qA166 amplicons before PCR is not necessary (B).