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. Author manuscript; available in PMC: 2011 Feb 23.
Published in final edited form as: Cancer Res. 2008 Apr 15;68(8):2878–2884. doi: 10.1158/0008-5472.CAN-07-6028

Figure 4.

Figure 4

In vivo imaging of transgene activity provides an index of biological enzyme function. (a) 19FMRS image and associated parametric map demonstrating circumscribed regions of CD-UPRT levels and activity within a CD-UPRT+/CD-UPRT- co-transplanted tumor xenograft at a digital resolution of 1.8 mm × 1.8 mm. (b) The regional distribution of UPRT activity closely parallels the expression pattern of the CD-UPRT transgene as determined by immunohistochemical staining. Apoptotic indices measured in a contiguous slice of tumor tissue through staining for cleaved capase-3 demonstrated that regions with low, medium and high apoptotic indices (c, d, e) correlated well with transgene activity, (13.2 apoptotic cells/mm2 ± 2.9, 23.8 cells/mm2 ± 1.8 and 61.2 apoptotic cells/mm2 ± 2.4 respectively, P<0.0001).