Table 2.

Effect of choline ± methionine deficiency on liver weights and TGs, adipose depot weights, and food intake

	CS	CDD	MCDD
Liver weight, %body weight	4.0 ± 0.1	4.7 ± 0.1***	3.8 ± 0.1†††
Liver TG pool, μmol/liver$	24.37 ± 2.4	45.94 ± 3.9***	43.30 ± 3.5***
Adipose depot weights, %body wt
Subcutaneous$	1.5 ± 0.2	1.3 ± 0.1	0.7 ± 0.1***††
Epididymal$	1.6 ± 0.1	1.4 ± 0.1	0.4 ± 0.0***†††
Mesenteric§	0.3 ± 0.1	0.4 ± 0.1	0.1 ± 0.0*†††
Food intake, g·kg body wt−1·day−1§	127.9 ± 4.5	130.8 ± 2.8	104 ± 2.7***†††
Liver function tests§
Bilirubin, μmol/l	4.9 ± 0.8	4.5 ± 0.7	5.3 ± 0.6
ALT, U/l	31 ± 3	48 ± 4	231 ± 79†*
AST, U/l	303 ± 86	224 ± 22	507 ± 90†

Data are means ± SE, analyzed by 1-way ANOVA with Tukey's post hoc tests where appropriate. TG, triglyceride; ALT, alanine aminotransferase; AST, aspartate aminotransferase.

^*P < 0.05,

^***P < 0.001 vs. CS;

^†P < 0.05,

^††P < 0.01, and

^†††P < 0.001 vs. CDD. Data were collected in different experiments and combined here, hence the varying numbers; $n = 21–23/group,

^§n = 7–8/group. For all other variables, n = 28–30/group.