Fig. 3.

Hepatic mRNA levels for Pgc1α (A), Pck1 (B), G6pc (C), Pdk4 (D), and Gck (E) genes were analyzed in control loxp, LKO^Sirt1, DKO^Irs1/2, and TKO^Irs1/2:Sirt1 mice by real-time RT-PCR after being fasted overnight for 16 h with free access to water or refed ad libitum for another 4 h immediately after fasting. Values are presented as means ± SE; n = 3. Protein levels for Pgc-1α, Pck1, Pdk-4, Gck, and the internal control actinin (F) were also analyzed in the above fasted and refed liver lysates.