Glucose is reduced and lactate is elevated in EOMA-conditioned medium, but exogenous glucose or lactate have no effect on PLGF mRNA expression. A: glucose was consumed and lactate was produced by EOMA cells during the conditioning phase, resulting in decreased glucose levels and increased lactate levels in conditioned media (n = 7) relative to fresh media (n = 5). *P < 0.001 (t-test); **P < 0.01 (Mann-Whitney rank sum test). ND, not detected. B: exogenous glucose (0–20 mM, 4 h) had no effect on PLGF gene expression by A10 SMC (n = 3/group). P = NS for all (ANOVA). C: exogenous lactate (0–10 mM, 4 h) had no effect on PLGF gene expression by A10 SMC (n = 3–5/group). P = NS for all (ANOVA).