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. 2010 Dec 1;300(2):C368–C374. doi: 10.1152/ajpcell.00314.2010

Fig. 4.

Fig. 4.

Effect of soluble adenylyl cyclase (sAC) small interfering RNA (siRNA) on basal and SP-induced apoptosis. A, top: sAC expression and siRNA transfection under BF and BR conditions. Nontransfected cells (NT), cells transfected with scrambled control siRNA sequences (siCon), and cells transfected with sAC-specific siRNA (siRNA) were analyzed for sAC expression (50 kDa) relative to β-actin by Western blot 48 h following transfection. Bottom: densitometric analysis of sAC expression normalized to β-actin and plotted relative to BF no treatment. Values are means ± SE; n = 3. *Significantly less sAC expression (P < 0.05 by paired t-test). B: flow cytometric analysis (annexin V+ cells) of endothelial cells incubated in BF or BR media for 48 h following transfection with siCon or sAC siRNA and treated with SP. Values are means ± SE; n = 7. *Significantly different from NT; ^ significantly different from siCon; + significantly different from siCon + SP: P < 0.05.