Table 1.
Ten DNA methylation sites identified as significantly associated with chronological age in all tissues from stage I
| Name | Chr | Genomic position (bp) | Symbol | Distance to TSS | Stage I P-value | Stage II P-value | Beta coefficient range | Adjusted r2 estimates from stage I | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CRBLM | FCTX | PONS | TCTX | CRBLM | FCTX | CRBLM | FCTX | PONS | TCTX | ||||||
| cg06144905 | 17 | 24393906 | PIPOX | 138 | 7.3 × 10−7 | 6.2 × 10−13 | 3.4 × 10−7 | 1.1 × 10−18 | 5.8 × 10−8 | 3.2 × 10−18 | 1.5 × 10−3, 3.4 × 10−3 | 0.3085 | 0.4424 | 0.3524 | 0.627 |
| cg06993413 | 15 | 63597257 | DPP8 | 162 | 2.7 × 10−10 | 5.4 × 10−16 | 9.0 × 10−8 | 5.4 × 10−14 | 5.5 × 10−21 | 3.8 × 10−10 | 4.3 × 10−4, 9.8 × 10−4 | 0.5725 | 0.6796 | 0.3776 | 0.5975 |
| cg10523019 | 2 | 227408702 | RHBDD1 | 315 | 7.7 × 10−7 | 9.2 × 10−9 | 6.1 × 10−15 | 3.2 × 10−11 | 2.0 × 10−16 | 5.5 × 10−15 | 5.9 × 10−4, 1.2 × 10−3 | 0.5949 | 0.6909 | 0.6369 | 0.6641 |
| cg14424579 | 2 | 27127813 | FLJ21839 | 240 | 5.9 × 10−7 | 3.5 × 10−8 | 3.2 × 10−7 | 3.7 × 10−14 | 2.5 × 10−15 | 9.4 × 10−12 | 2.8 × 10−4, 4.0 × 10−4 | 0.6488 | 0.7015 | 0.5533 | 0.6195 |
| cg15201877 | 1 | 71285561 | PTGER3 | 518 | 5.4 × 10−10 | 1.3 × 10−14 | 1.1 × 10−13 | 1.6 × 10−18 | 5.1 × 10−8 | 8.5 × 10−26 | 3.4 × 10−4, 1.6 × 10−3 | 0.5928 | 0.7205 | 0.6902 | 0.8032 |
| cg18108623 | 17 | 30725434 | FLJ34922 | 701 | 1.7 × 10−6 | 9.3 × 10−12 | 1.8 × 10−7 | 9.6 × 10−18 | 3.1 × 10−8 | 1.2 × 10−22 | 3.0 × 10−4, 8.4 × 10−4 | 0.4937 | 0.717 | 0.6566 | 0.7531 |
| cg18555440 | 11 | 17698263 | MYOD1 | 528 | 1.8 × 10−6 | 2.2 × 10−8 | 3.6 × 10−7 | 2.6 × 10−10 | 1.0 × 10−26 | 6.0 × 10−11 | 5.7 × 10−4, 8.0 × 10−4 | 0.3153 | 0.5847 | 0.3713 | 0.6351 |
| cg19945840 | 1 | 1157899 | B3GALT6 | 391 | 4.2 × 10−10 | 1.7 × 10−6 | 1.8 × 10−12 | 5.0 × 10−10 | 6.9 × 10−20 | 2.3 × 10−17 | 1.9 × 10−3, 2.6 × 10−3 | 0.4704 | 0.3802 | 0.5503 | 0.5233 |
| cg21589115 | 19 | 54558926 | DKKL1 | 72 | 1.2 × 10−6 | 1.7 × 10−12 | 6.2 × 10−15 | 2.4 × 10−16 | 5.1 × 10−13 | 3.2 × 10−26 | 5.8 × 10−4, 1.8 × 10−3 | 0.5176 | 0.6528 | 0.588 | 0.7055 |
| cg27529628 | 12 | 99491350 | GAS2L3 | 270 | 8.1 × 10−15 | 1.8 × 10−16 | 8.2 × 10−12 | 7.8 × 10−26 | 2.3 × 10−27 | 3.8 × 10−26 | 7.1 × 10−4, 1.5 × 10−3 | 0.6565 | 0.7556 | 0.6507 | 0.812 |
Notably, the DNA methylation level at each of these CpG sites is significantly and consistently associated with chronological age in stage II. CRBLM, cerebellum; FCTX, frontal cortex; PONS, pons; TCTX, temporal cortex. Genomic position is based on hg18 of the human genome.