Fig. 4.

Semiquantitative immunocytochemistry of aquaporin-2 (AQP2). AQP2-labeled mouse cortical collecting duct cells (M-1 cells) were randomly selected (asterisk in A) and total pixel intensity of the plasma membrane (PM) (C) and whole cell (both PM and cytosol except nucleus, D) were acquired respectively from the selected cells (B). E and F) AQP2 labeling in M-1 cells transfected with Con-small interfering RNA (Con-siRNA) or AS160-siRNA. G) The ratio of total pixel intensity in the PM to that of the whole cell (ratio of PM/Total) was calculated from randomly selected M-1 cells and averaged in each group. N, nucleus; n = number of cells randomly chosen and examined in each group.

^*P < 0.05 when compared to Con-siRNA. Bar, 10 µm.