Medullary tissue monoamine levels detected by high-performance liquid chromatography (HPLC) in P15 rat pups from dams fed a control or a tryptophan-deficient diet; n = 5 males and females in each group. Significance tested by t-test: **P < 0.01, ***P < 0.001. DOPAC, 3,4-dihydroxyphenylacetic acid, a dopamine (DA) metabolite; 5-HIAA, 5-hydroxyindoleacetic acid, a serotonin (5-HT) metabolite; HVA, homovanillic acid, a catecholamine metabolite.