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. 2010 Oct 21;110(2):318–328. doi: 10.1152/japplphysiol.00788.2010

Fig. 7.

Fig. 7.

Tryptophan-deficient diet had no detectable effect on tryptophan hydroxylase (TPH) immunolabeling but decreased the intensity of perisomatic 5-HT immunolabeling in males. A: average intensity of TPH immunolabeling per cell of individual tryptophan-deficient pups expressed as % relative to pair-matched control pups. Each symbol represents the values for 1 animal, with the horizontal bars marking the group average. There was no detectable effect of the diet exposure on TPH immunolabeling in males (M) or females (F), and groups averaged 96% and 100% of controls, respectively. A′ and A″ show representative TPH immunolabeling in the raphé magnus from pair-matched control male (cM, A′) and tryptophan-deficient diet-exposed male (dM, A″). In both cases, TPH-labeled cells (arrows) are clearly visible. B: intensity of perisomatic 5-HT immunolabeling following tryptophan-deficient diet exposure decreases on average to 65% of control values in males (raw intensity values compared with control by t-test: *P < 0.05). Intensity of 5-HT immunolabeling in females was more variable and averaged 96% control values. B′ and B″ show immunolabeling for 5-HT (arrows) in the same cells as A′ and A″. Cell bodies are clearly visible in male control tissue (cM, B′) but less distinct in tissue from tryptophan-deficient diet-exposed male tissue (dM, B″). Scale bar, 30 μm.