In vitro isolated renal pelvises, low-sodium diet. A: effects of 1,250 pM NE on the release of substance P and PGE2 in the presence of vehicle (solid line) or 0.1 μM rauwolscine (dashed line) in the bath. B: effects of 250 pM NE on the release of substance P and PGE2 in the presence of rauwolscine (dashed line) or rauwolscine+losartan (dotted line) in the bath.**P < 0.01, vs. CNT and REC; ‡P < 0.01 NE-induced substance P and PGE2 release in the presence of vehicle vs. rauwolscine (A) and rauwolscine vs. rauwolscine+losartan (B). CNT, control; REC, recovery.