Fig. 1.

Brain regions harvested for molecular experiments and location of slices for electrophysiological recordings. Cortical samples (A), medullary samples (B), and punches containing the ventral respiratory column (VRC; B) were used for PCR and Western blot. Slices taken through the forebrain (A; hatched area) and through the medulla (B) were used for electrophysiological recordings. Solid gray bars indicate a sample location of coronal sections used for immunohistochemistry. IV, fourth ventricle; NTS, nucleus of the solitary tract; BötC, Bötzinger complex; Pre-BötC, pre-Bötzinger complex; rVRG, rostral ventral respiratory group; cVRG, caudal ventral respiratory group; NA, nucleus ambiguus; XII, hypoglossal nucleus.