Fig. 1.

Representative iTRAQ spectra. Isolated mitochondrial subpopulations from control and diabetic hearts were labeled with iTRAQ reagents 114 [diabetic subsarcolemmal mitochondria (SSM)], 115 [diabetic interfibrillar mitochondria (IFM)], 116 (control SSM), and 117 (control IFM); then, they were combined for analysis with mass spectrometry. A: representative spectra of simultaneous quantitation of a mtHsp70 peptide in control and diabetic mitochondrial subpopulations. B: MS/MS spectra for the reporter groups of the iTRAQ reagents (114, 115, 116, and 117) from a mtHsp70 peptide. These spectra were used along with other peptides to simultaneously quantify mtHsp70 control and diabetic mitochondrial subpopulations.