Fig. 2.

Representative two-dimensional-differential in-gel electrophoresis (2D-DIGE) gels. Samples were labeled with either Cy3 or Cy5, alternating between control and diabetic SSM and IFM. A control and treated mitochondrial sample was run on the same gel, and an internal standard was labeled with Cy2 and run on every gel for gel-to-gel comparisons. A: representative gel showing Cy3-labeled control IFM. B: same gel as A showing Cy5-labeled diabetic IFM. C: representative gel showing Cy3-labeled control SSM. D: same gel as C showing Cy5-labeled control SSM. Below the gel images are individual spots that were identified as mtHsp70 (indicated by the red arrow), and quantitative differences were assessed by examination of peak density.