Fig. 4.

Mitochondrial protein import. Effect of type 1 diabetes mellitus on MitoGFP1 import in SSM and IFM subpopulations. Cardiac mitochondrial subpopulations from control and diabetic mice were isolated and incubated with 3 μl of MitoGFP1 protein lysate at 2-, 5-, and 10-min time points. Representative Western blots and fluorescent images from SSM (A) and IFM (B) protein import assay. Graphical representation of mitochondrial protein import performed in SSM (C) and IFM (D) control and diabetic subpopulations. The relative amount of imported MitoGFP1 was determined by densitometry. Values were calculated based upon a 100% scale, and presented as mean ± SE; *P < 0.05 for control vs. diabetic subpopulations. Control for protein loading was confirmed by Ponceau staining; n = 8 per group.