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. 2010 Dec 3;300(2):L295–L304. doi: 10.1152/ajplung.00134.2010

Fig. 7.

Fig. 7.

Effect of Nox4 small-interfering RNA (siRNA) on the regulation of MnSOD, catalase, and IL-6 expression by TGF-β. AMSCs were transfected with Nox4 siRNA (250 nM) for 18 h, serum deprived for 6 h, and then incubated with TGF-β (1 ng/ml) for 48 h. Nox4 mRNA was determined by real-time PCR (A), and protein was determined in whole cell extracts by Western blotting (B). MnSOD, catalase, and IL-6 (C) mRNA were determined by real-time PCR. mRNA data were expressed as fold change with respect to unstimulated control. Bars represent means ± SE of 4 ASMC donors. *P < 0.05 and **P < 0.01 compared with unstimulated control; #P < 0.05 compared with TGF-β (1 ng/ml)-stimulated cells.