Doxorubicin does not alter diaphragm TNF mRNA or protein. A: TNF mRNA in the diaphragm following doxorubicin administration (24 h: vehicle n = 5, treated n = 6; 48 h: vehicle n = 5, treated n = 5; 72 h: vehicle n = 6, treated n = 6). B: TNF protein with representative lanes (24 h: vehicle n = 3, treated n = 3; 48 h: vehicle n = 3, treated n = 3; 72 h: vehicle n = 3, treated n = 3). Data are expressed as % change from time-matched control values.