Table 1. Sequences of the primers and probes used in quantitative PCR studies.
Gene | Oligonucleotide | Sequence | PCR product size (bp) | GenBank Accession Number |
---|---|---|---|---|
YWHAZ | Forward primer | 5′-CCAATGCTTCACAAGCAGAGAGCA-3′ | 99 | NM_003406 |
Reverse primer | 5′-CTTTCTTGTCATCACCAGCGGCAA-3′ | |||
Probe | 5′-fAGGAGATTACTACCGTTACTTGGCTGAGGq-3′ | |||
RRM2 | Forward primer | 5′-TTTAGTGAGCTTAGCACAGCGGGA-3′ | 89 | NM_001034 |
Reverse primer | 5′-AAATCTGCGTTGAAGCAGTGAGGC-3′ | |||
Probe | 5′-rACAGTCCTTTAACCAGCACAGCCAGTq-3′ |
f denotes the fluorescein FAM, r denotes the fluorescein TET, q denotes Black Hole Quencher 1