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Actin distribution in primary human fibroblasts on 2D and within 3D matrices (A) and structure of 3D matrices (B). Images of actin in 3D matrices represent a single z slice through the central region midline of each cell. 3D CDM was stained using an antibody to fibronectin. Collagen and BME were visualized using reflection microscopy. Fluorescently labeled fibrinogen was used to visualize the fibrin matrices. Images were acquired using a confocal microscope. Scale bar represents 10 μm.
