FIG. 4.

Velocity and directionality of fibroblasts migrating in 2D and 3D matrices. Imaging was initiated at 24 h after adding human fibroblasts to matrices. The data were collected by tracking movements of 20–60 cells under each condition in at least three separate experiments. Velocity (A) and directionality (B) were calculated using MetaMorph and Excel. The concentrations of 3T3 CDM, BME, collagen, and fibrin in the matrices were 5.2, 10, 2.5, and 4 mg/mL, respectively. Bars represent standard error. *p < 0.05, **p < 0.01, and ***p < 0.001.