FIG. 5.

Distribution of β₁ integrin and F-actin in 2D and 3D matrices. Human fibroblasts were cultured on 2D matrix-coated glass coverslips (A) or in 3D matrices (B) and fixed after 24 h of culture. Samples were stained for activated β₁ integrin (monoclonal antibody 9EG7, green) and F-actin (phalloidin, red). Images are maximum projections of z slices, unless otherwise indicated. Representative images are shown from three separate experiments. Scale bar represents 10 μm.