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Distribution of vinculin and F-actin in 2D and 3D matrices. Fibroblasts were cultured on 2D matrix-coated glass coverslips (A) or in 3D matrices (B) and fixed after 24 h of culture. Samples were stained for vinculin (green) and F-actin (phalloidin, red). Images are maximum projections of z slices. Representative images are shown from three separate experiments. Scale bar represents 10 μm.
