Fig. 6.

TrkA IR in urothelium (U) (A–F) and detrusor smooth muscle (sm) (C, F) of littermate WT (A–C) and NGF-OE (D–F) mice. TrkA IR was significantly (P ≤ 0.01) reduced in urothelial cells of NGF-OE mice (A–F). TrkA IR was also significantly (P ≤ 0.01) reduced in detrusor sm of NGF-OE mice (C, F). G: higher-power image of TrkA IR in urothelial cells in littermate WT mice. Trk staining was visualized with Cy3-conjugated secondary antibody (red areas). YOYO-1 identified nuclear profiles of urothelial cells and was visualized with Cy2-conjugated secondary antibody (green areas). L, lumen. Calibration bar: 50 μm (B, E), 125 μm (A, C, D, F), 30 μm (G). H: summary histogram of TrkA expression in U and detrusor sm in WT and NGF-OE mice. Values are means ± SE for n = 5–7. *P ≤ 0.01.