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. 2010 Nov 10;300(2):F581–F588. doi: 10.1152/ajprenal.00433.2010

Fig. 2.

Fig. 2.

Renin immunoreactivity in juxtaglomerular (JG) cells and collecting duct (CD) cells of paraffin-embedded kidney sections of Cyp1a1Ren2 rats with inducible ANG II-dependent malignant hypertension and noninduced normotensive rats. Top panels show similar renin-positive immunoreactivity (3,3′-diaminobenzidine tetrahydrochloride, brown chromogen; arrow) in the JG cells of a noninduced rat (A) and a Cyp1a1Ren2 rat (B) kidney sections (3 μm) stained by immunoperoxidase technique. Likewise, C (noninduced rat) and D (Cyp1a1Ren2 rat) show renin immunoreactivity in CD cells. Semiquantitation of renin immunoreactivity (E) in JG cells is not different between noninduced and Cyp1a1Ren2-hypertensive rats; however, renin immunoreactivity in CD cells is 3.8-fold higher in hypertensive rats than in noninduced rats. F: representative renin and prorenin Western blot and densitometry analysis using loads of 40 μg/lane of protein extracted from renal medullary tissue samples from noninduced and Cyp1a1Ren2-hypertensive rats induced with 0.3% high indole-3-carbinol (I3C). As positive controls, 1 μg of human recombinant prorenin (hPR) and human recombinant renin (hR) was used. MW, molecular weight. Values are means ± SE. *P < 0.05 vs. noninduced rats.