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. 2010 Dec 3;300(2):H448–H458. doi: 10.1152/ajpheart.01178.2009

Fig. 1.

Fig. 1.

A: activity of RhoA (as RhoA-GTP) and Rho kinase (ROCK) in porcine valvular interstitial cells (VICs) on day 5 of culture in different environments. Fibrin (FB) and tissue culture polystyrene (TCPS) represent environments that support nodule formation, whereas fibronectin (FN) and collagen (Coll) represent environments that do not. *P < 0.05 compared with Coll. B: ROCK activity in VIC cultures at two earlier time points, days 1 and 3. n = 4 samples/condition. *P < 0.05 compared with both Coll and FN on day 3. C: ROCK activity in VIC cultures treated with the RhoA stimulant lysophosphatidic acid (LPA) or the ROCK inhibitors H1152 and Y27632 (day 5). *P < 0.01 compared with the untreated TCPS condition. D: impact of direct Rho activation (via administration of LPA) on the formation of nodules in VIC cultures. LPA was also administered in the presence of a ROCK inhibitor (H1152) to further understand the contribution of Rho to nodule formation. n = 4 samples/condition. *P < 0.04 compared with the untreated condition; ^P < 0.05 compared with the LPA-treated condition.