Table 2.
AHL production by E. coli expressing either splI or spsI from G3
| AHL produced by G3 WT[23] | AHL expressed in E. coli/splI# | AHL expressed in E. coli/spsI# |
|---|---|---|
| C4-HSL | + | ++++ |
| C5-HSL | + | +++ |
| C6-HSL | ++ | ++ |
| C7-HSL | ++ | + |
| C8-HSL | + | + |
| 3-oxo-C6-HSL | +++ | - |
| 3-oxo-C7-HSL | ++ | - |
| 3-oxo-C8-HSL | + | - |
| 3-hydroxy-C6-HSL | ++ | - |
| 3-hydroxy-C8-HSL | + | - |
AHL profiles identification was performed by LC-MS/MS from two independent experiments.
# AHL mass abundance (relative quantity of íons from a particular AHL relative to that of a known standard) on LC-MS/MS: ++++ indicates 107; +++ indicates 106; ++ indicates 105; + indicates ≤104.