Figure 2.

qRTPCR analysis of FGF-2 regulated genes associated with mineralization; qRTPCR analysis of gene reduction of genes over a 24 hours of treatment with FGF-2 shows a marked reduction in genes associated with mineralization. Cultures were cultured and harvested for RNA as described in Materials and Methods. Each bar represents mean ± SD triplicate independent biological samples at each time point corrected to cyclophilin. (*p < 0.05; **p < 0.01 with two-tail student t-test compare to 0 hour of each gene.)