Table 3.

Mineralization of cells with BMP-2

Treatment	Relative abundance
NM	5.6 ± 1.7
NM + 5 ng/ml FGF-2	5.3 ± .1
NM + 50 ng/ml BMP-2	16.2 ± 4.2
MM	9.1 ± 2.0
MM + 5 ng/ml FGF-2	4.9 ± 1.1
MM + 50 ng/ml BMP-2	55.2 ± 12.7

The Alizarin Red (2%) stained cells were incubated with 10% acetic acid for 30 minutes to release bound Alizarin Red into solution. The solution was neutralized with 10% ammonium hydroxide and the absorbance of Alizarin Red was measured at 450 nm using a microplate reader (n = 6). Data is expressed at in absolute amounts according to a standard curve.