Table 1.
mRNA Levels of extracellular matrix (ECM) molecules in cultured discoidin domain receptor 2 (DDR2)+/+ and DDR2-/- skin fibroblasts
| mRNA | DDR2+/+ | DDR2-/- | Expression when DDR2 is absent |
|---|---|---|---|
| Col I (α 1) | 2.54 ± 0.04 | 1.34 ± 0.11 | Down |
| Col VI (α 2) | 0.31 ± 0.07 | 0.45 ± 0.05 | Up |
| Col VIII (α 1) | 0.56 ± 0.02 | 0.34 ± 0.05 | Down |
| Syndecan1 | 1.01 ± 0.06 | 0.06 ± 0.10 | Down |
| Fibrillin 1 | 0.95 ± 0.01 | 0.78 ± 0.02 | Down |
| Fibronectin | 0.17 ± 0.01 | 0.13 ± 0.04 | NS |
| α2 | 0.92 ± 0.03 | 1.52 ± 0.03 | Up |
| α3 | 0.67 ± 0.07 | 0.61 ± 0.04 | NS |
| DDR1 | 0.94 ± 0.20 | 0.91 ± 0.38 | NS |
| MMP2 | 0.94 ± 0.01 | 0.31 ± 0.08 | Down |
| MMP9 | ND | ND | ND |
| MMP10 | 0.57 ± 0.09 | 0.24 ± 0.03 | Down |
| MMP13 | 1.34 ± 0.10 | 1.17 ± 0.09 | NS |
Relative mRNA expression of ECM molecules regulated by DDR2 expression in DDR2+/+ and DDR2-/- skin fibroblasts. Gene expression was normalized to RPL13 mRNA expression. ND indicates mRNA was not detected. Results are expressed as means ± standard error of the mean (SEM) (n = 3). All P < 0.05 except for NS.
MMP = matrix metalloproteinase; NS = not significantly different.