Figure 1.

Evidence for an export motif in polycystin-2. COS-7 cells were transiently transfected with expression plasmids for the polycystin-2 mutant proteins indicated. 2 d later the cells were stained with an antibody directed against an extracellular domain (loop 1) of polycystin-2 (red) in the presence and absence of the detergent Triton X-100. Only when the mutant proteins reached the plasma membrane could a signal be detected in the absence of Triton X-100. Nuclei were counterstained with Hoechst 33258 (blue) to demonstrate the presence of cells. Bar, 100 µm.