Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Feb 21;192(4):663–674. doi: 10.1083/jcb.201008140

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Tsun et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — Lck is essential for degranulation and lytic granule polarization toward the immunological synapse. (A) Merged immunofluorescence images of three lytic granule polarization phenotypes seen in F5 CTL conjugates with lytic granules: (i) >90% of granules distal, (ii) dispersed, or (iii) tightly clustered at the synapse. Conjugates are labeled with antibodies against LAMP1 (red) or CD8 (white). Nuclei are stained with Hoechst (blue). (B) Representative images of granule polarization observed in (i) WT and (ii) Lck^off F5 CTL conjugates. (C) Quantitation of granule polarization in F5 WT (n = 465) and Lck^off (n = 484) CTLs for granules that were distal (blue), dispersed (red), or tightly polarized (green) at the immune synapse. Error bars show the standard deviation from the means of at least three independent experiments. A two-tailed Student’s t test for loss of granule polarization in Lck^off samples compared with WT gave a statistical significance of P = 5 × 10⁻⁶. (D) Histograms of a CTL degranulation assay displaying a CD8⁺ cell count against a LAMP1-PE signal upon activation with pulsed (shaded) or unpulsed (unshaded) EL4 targets. The percent increase in LAMP1 staining with a pulsed versus an unpulsed target is given. Data are representative of more than three independent experiments. Bars, 5 µm.