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. 2011 Feb 21;192(4):663–674. doi: 10.1083/jcb.201008140

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© 2011 Tsun et al.

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Figure 5. — Actin clearance into the dSMAC is impaired in the absence of Lck F5 WT and Lck^off CTL conjugates with NP68-pulsed EL4 targets. (A) Quantitation of forming dSMACs from 3D reconstructions labeled for actin (WT, n = 121 and Lck^off, n = 110). (B) Representative 3D images of actin staining in WT and Lck^off CTLs in which actin has failed to clear (ii) or has partially reorganized (iii). (C) Quantitation from 3D reconstructions (WT, n = 117 and Lck^off, n = 119). (D) 3D images for pSMACs labeled for talin at the synapse. All 3D images are displayed at 0, 45, and 90° rotations. Bars, 10 µm.