Table III.

Oligonucleotides

Oligos	Sequence	Purpose	Gene
FLO1	5′-CCATGTTTAA­CCGTCCGTGCA­TTAAATCCATT­CATGAAGAGG ­GTAGGTGGTCC­CGGTGGTCGGA­TCCCCGGGTTA­ATTAA-3′	Tagging	SSD1
FLO2	5′-AGTGAAAAAC­AAGAAAAACAG­CAATGACGATA­TTGG TAGAAGAGAGAATTCGAGCTCGTTTAAAC-3′	Tagging	SSD1
FLO203	5′-CTTTCAGCGC­AAAGATTTGGC­CCAATTATTCC­ATCTTTA TACACTCGGATCCCCGGGTTAATTAA-3′	Deletion	SSD1
FLO527	5′-GGGGACAAGT­TTGTACAAAAA­AGCAGGCTTCG­AAGG ­AGATAACAAAA­TGTCTAAAAAT­AGCAACGTTAACA-3′	Gateway cloning	SSD1
FLO536	5′-GGGGACCACT­TTGTACAAGAA­AGCTGGGTCTA­CCCT CTTCATGAATGGATTTAA-3′	Gateway cloning	SSD1
FLO560	5′-GTACAACACC­ACTTCGATTAC­TAATTCGACCA­GTTG GTGAACGCTGCAGGTCGACAACCC-3′	mRNA tagging	SRL1
FLO561	5′-AAACAAAAAG­AACTTAAAAGG­ACACGTTTGAA­CTCA TAATGCATAGGCCACTAGTGGATC-3′	mRNA tagging	SRL1
FLO588	5′-ATGAAATTCTCAACTGCC-3′	RT-PCR	SIM1
FLO589	5′-GGTGAAGTGGAAGAAGTACT-3′	RT-PCR	SIM1
FLO590	5′-ATGCAATTTTCTACTGTCGC-3′	RT-PCR	CCW12
FLO591	5′-ACAACAACAAAGCAGCG-3′	RT-PCR	CCW12
FLO618	5′-ATGAAACTGTCCGCCAC-3′	RT-PCR	SUN4
FLO619	5′-TGTAACTGCAACGGCCC-3′	RT-PCR	SUN4
FLO620	5′-ATGGGGAAACAGTCAAA-3′	RT-PCR	DSE2
FLO621	5′-CGACGGAATTAGCTAAA-3′	RT-PCR	DSE2
FLO622	5′-ATGCGTCTCTCTAACCTAATT-3′	RT-PCR	SCW4
FLO623	5′-TGAAGCGGCACCATTAA-3′	RT-PCR	SCW4
FLO624	5′-GGAACTTAAACTGATGT-3′	RT-PCR	CTS1
FLO625	5′-CAACCGATGCCTTTGAT-3′	RT-PCR	CTS1
FLO627	5′-TACGCCGACACCACTACCT-3′	qRT-PCR	SRL1
FLO628	5′-GTGGACGTGATAGTCGAGGTGGAG-3′	qRT-PCR	SRL1
FLO629	5′-GGTATCATGGTCGGTATGGGTCA-3′	qRT-PCR	ACT1
FLO630	5′-TGTTCTTCTGGGGAACTTCTCA-3′	qRT-PCR	ACT1
FLO631	5′-TGCGGACCCTTTTGCCAC-3′	qRT-PCR	CCW12
FLO632	5′-ATCCGGCGGTAGCGCAT-3′	qRT-PCR	CCW12
FLO633	5′-TTGGTGCTGGCTCCACTGG-3′	qRT-PCR	SIM1
FLO634	5′-ACACCCGTCAGCACCGC-3′	qRT-PCR	SIM1
FLO635	5′-TCCAAAGCCAAAGGCCAACG-3′	qRT-PCR	ADH1
FLO636	5′-CCGATCTTCCAGCCCTTAACG-3′	qRT-PCR	ADH1
FLO639	5′-ATGCTTCAATCCGTTGT-3′	RT-PCR	SRL1
FLO640	5′-CGTATTTGGGGTGACTG-3′	RT-PCR	SRL1