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. 2011 Feb 23;6(2):e17197. doi: 10.1371/journal.pone.0017197

Figure 2. Switching the PMA-containing M254 medium to PMA-free Mel-mix medium results in cell morphology changes and loss of c-Kit and TRP-1 expressions.

Figure 2

When melanocyte cultures were initiated in PMA-containing M254 medium, cells were dendritic, and expressed TRP-1 as well as c-Kit proteins (100% of the cells expressed TRP-1 and c-Kit). At week 4, when cells were in 4th passage, half of the cultures were switched to PMA-free Mel-mix medium. Cells in Mel-mix medium became bipolar (B), lost TRP-1 (D) and c-Kit (F), and increased EGFR (H) expressions in two weeks, while melanocytes continuously cultured in M254 medium remained dendritic (A) expressed both TRP-1 (C) and c-Kit (E) proteins, and showed only a week EGFR staining (G). Bar: 100 µm.