Figure 4. Melanocytes cultured in PMA-free Mel-mix medium proliferate more rapidly than melanocytes cultured in PMA-containing M254 medium.

CFSE measurements were performed on melanocytes cultured in Mel-mix and M254 medium. Based on CFSE peak fluorescence intensity halving, cell division numbers for melanocytes cultured in Mel-mix medium (solid line) was 8, while for cells cultured in M254 medium (dashed line) was only 4.5 in the 120-hour experimental period.