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. 2011 Feb 23;6(2):e17197. doi: 10.1371/journal.pone.0017197

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Kormos et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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β-galactosidase assay was performed to detect senescent cells (blue cells) in the cultures. 3^rd passage melanocytes cultured in Mel-mix medium (A), 3^rd passage melanocytes cultured in M254 medium (B), 8^th passage melanocytes cultured in Mel-mix medium (C), 8^th passage melanocytes cultured in M254 medium (D). Bar: 100 µm.