Skip to main content
. 2011 Feb 23;6(2):e17379. doi: 10.1371/journal.pone.0017379

Figure 2. The migratory epithelioid cells are of post-mitotic neuronal character.

Figure 2

(A) A DAPI transversal section of a stage 19 forebrain, showing the location of the olfactory pit (OP) in correlation to the telencephalon (T). At stage 17, 19, 22 and 27 HuC/D expression is detected in migratory cells emanating from the olfactory epithelium. (B) Stage 12/13 chick embryos were electroporated in ovo in the olfactory placodal region using a construct encoding GFP, and cultured to approximately stage 19. Embryos with GFP expression in the olfactory pit region were selected, sectioned and analysed. All GFP+ migratory cells express HuC/D (MN), and the migratory HuC/D+ neurons express Ngn1, but not Hes5 (arrowheads). Expression of HuC/D, Ngn1 and Hes5 can be detected in the olfactory epithelium (OE).