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. 2011 Feb 23;6(2):e17379. doi: 10.1371/journal.pone.0017379

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Maier et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A, B) In ovo electroporation of stage 12/13 in the olfactory placodal region using a GFP construct together with a dnMAMLI or a caNotch1 construct and cultured to stage 22. (A) In dnMAMLI-electroporated olfactory sensory epithelium, Hes5 expression was reduced, but pSmad1/5/8 expression was unaffected, compared to the non-electroporated side. (B) In caNotch1-electroporated lateral part of the olfactory pit and surface ectoderm, Hes5 expression was induced, but pSmad1/5/8 expression was unaffected, in the most compared to the non-electroporated side.