Figure 6. ACT-induced decrease of macrophage adhesion.

Treatment with 35 nM ACT for 10 min reduces J774A.1 cell adhesion. The effect of the toxin on adhesion was partially reduced by pre-treatment with nifedipine (100 µM), a Ca²⁺ channel inhibitor, KT5720 (56 nM), a PKA inhibitor and with calpeptin (50 µM), an inhibitor of the protease calpain. All molecules were pre-incubated for 30 min with J774A.1 cells before the addition of ACT. Adhesion was measured as described in Materials and Methods; the data shown is the mean ± SEM of at least three independent experiments.