TABLE 2.

Effects of TCDD on Immune Cells and Their Regulatory Cell Surface Marker Expression during TNBS-Induced Colitis in Foxp3^egfp Mice

	MLNs			Colon
	Vehicle	TCDD	p Value	Vehicle	TCDD	p Value
A. Tregs in the gut
CD4+CD25+Foxp3+	6.34	7.74	0.036	26.8	15.98	0.017
CTLA4	11.78	12.30	0.411	4.05	13.80	0.001
GITR	83.86	85.52	0.274	386	710	< 0.0001
CD103	45.36	51.90	0.008	1945	2072	0.104
α4β7	18.12	20.04	0.263	235	263	0.029
CD4+CD25−Foxp3+	3.90	3.98	0.444	16.78	17.02	0.445
CTLA4	2.20	2.94	0.151	0.62	1.42	0.033
GITR	78.66	78.88	0.472	325	458	0.005
CD103	32.90	42.74	< 0.0001	1639	1915	0.007
α4β7	5.60	8.36	0.009	155	185	0.013
B. DCs in the gut
CD11c+	3.43	2.74	0.036	9.56	11.65	0.030
MHC2	81.73	82.4	0.369	20.56	11.07	0.001
CD86	652	592	0.228	338	364	0.288
CD103	24.43	33.14	0.005	88.54	92.43	0.160
MHC2+CD103+	8.673	11.88	0.024	6.595	4.910	0.089

Leukocytes from colon tissue and MLNs were isolated 3 days after disease induction and immunophenotypically evaluated by flow cytometry to determine the presence of Tregs and DCs. Values are listed as the percentage of the bolded parent population indicated in the table, except for GITR, CD103, and α4β7 expression on colon T cells and CD86 expression on DCs, which indicate the median fluorescence intensity values. Bolded p values indicate significance of p ≤ 0.05. Data are representative of a single experiment (n = 6). Representative histograms are shown in Supplementary figures 1–3.