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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Reprod Toxicol. 2010 Oct 16;31(3):344–350. doi: 10.1016/j.reprotox.2010.10.003

Table 1.

Impact of Developmental TCDD Exposure on Reproductive Outcomes in Adult C57bl/6 Mice During a Mouse Parvovirus Outbreak

Mouse Exposure History generation Pregnancy Rate Pregnancy Outcome
Full-term Preterm
Vehicle Control1
 conF1 15/15 (100%) 15/152 0/15
 conF2 10/10 (100%) 10/10 0/10
 conF3 8/8 (100%) 8/8 0/8
 conF4 12/12 (100%) 12/12 0/12
TCDD in utero3
 F1 7/16 (44%) 1/7 6/7
 F2 3/7 (43%) 2/3 1/3
 F3 5/9 (55%) 3/5 2/5
TCDD in utero + prepubertal4
 F1 10/15 (66%) 4/11 7/11
 F2 2/7 (29%) 1/2 1/2
 F3 3/6 (50%) 2/3 1/3
 F4 6/8 (75%) 5/6 1/6
TCDD in utero + prepubertal + puberty5 0/5 (0%) N/A
1

Pregnant mice were exposed to corn oil vehicle (control) on E15.5 and control offspring (conF1 mice) mated at 10–12 weeks of age. Offspring of conF1 mice (conF2 mice) were mated at a similar age, as were the conF3 and conF4 generations of mice.

2

Only a subset of control F1–F3 offspring were used to obtain additional generations of unexposed mice.

3

Pregnant mice were exposed to 10 ug/kg TCDD in corn oil vehicle on E15.5 and singly exposed offspring (F1 mice) were mated at 10–12 weeks of age. Offspring of F1 mice (F2 mice) were mated at a similar age, as were the F3 generation.

4

Pregnant mice were exposed to 10 ug/kg TCDD in corn oil vehicle on E15.5 and offspring were additionally exposed to 10 ug/kg TCDD in corn oil vehicle, resulting in dually-exposed F1 mice (F1); mice were mated at 10–12 weeks of age. Offspring of F1 mice (F2 mice) were mated at a similar age, as were the F3 and F4 generations of mice.

5

Pregnant mice were exposed to 10 ug/kg TCDD in corn oil vehicle on E15.5 and offspring were additionally exposed to 10 ug/kg TCDD in corn oil vehicle at 4 weeks of age and at 9 weeks of age, resulting in triply exposed F1 mice (F1). Adult mice were mated at 10–12 weeks of age; no pregnancies were observed.