Fig. 3.

Protein levels for AXR1 and AXL1 are similar in axr1-30 transgenic lines, but only AXR1 line 1 shows moderate correction of axr1-30 phenotype in seedlings. a Protein levels in 8-day old seedlings. Based on anti-MYC immunoblot analyses, 10MYC-AXL1 (lanes 1–4) and 10MYC-AXR1 (lanes 5 and 6) accumulate protein to similar levels above background (lane 7), in characterized axr1-30 transgenic lines. 150 μg total protein was loaded for all samples. b Root length in 9-day old seedlings. Transgenic lines were plated on GM agar and grown for 9 days with germination marked on day 2. On day 9, seedlings were removed from plates, photographed, and root length was measured. Student’s t-tests with Bonferonni adjustment (α = 0.00385) were performed on combined data from three replicates. Plant lines marked with an asterisk are significantly different from axr1-30. Error bars represent SE with minimally n = 41 measurements per line