Table 1.
RUB conjugation components recovered by LC–MS/MS analysis of 3HA-RUB1 immunoprecipitations
| Gene | Uniprot ID | AGI | Mass (Da) | Unique peptidesa | Total unique hits | Protein ID probabilityb (%) | % Coveragec |
|---|---|---|---|---|---|---|---|
| AXL1 | Q9ZV69 | At2g32410 | 57924.1 | 12 | 63 | 100 | 33 |
| AXR1 | P42744 | At1g05180 | 60018.1 | 20 | 212 | 100 | 56 |
| CUL1 | Q94AH6 B9DGE3 | At4g02570 | 86287.4 | 7 | 11 | 100 | 12 |
| CUL4 | Q8LGH4 | At5g46210 | 91457.7 | 3 | 5 | 100 | 5 |
| ECR1 | O65041 | At5g19180 | 50524.4 | 18 | 264 | 100 | 48 |
| RCE1 | Q9SDY5 | At4g36800 | 20768.9 | 4 | 45 | 100 | 28 |
| RUB1 | Q9SHE7 | At1g31340 | 8872.8 | 3 | 460 | 100 | 50 |
aPeptides are designated as unique if they differ in primary amino acid sequence and satisfy rules of parsimony
bProtein ID probability is for the highest probability observed in 5 biological replicates
c% coverage is cumulative for 5 biological replicates