Fig. 6.

ABI4 binds to promoter fragments lacking any characterized ABI4 binding site. Electrophoretic mobility shift assays (EMSAs) with promoter fragments from At1g32560 (310 bp), At1g27470 (329 bp), At4g16160 (295 bp), At4g25140 (266 bp), At2g25890 (301 bp), At3g17520 (207 bp) or ABI4 (152 bp). The motifs contained within these fragments are indicated schematically. a Comparison of binding by GST versus GST-ABI4 DNA binding domain fusion demonstrates interaction with the ABI4 domain. b Competition with 50- or 100-fold molar excess of nonradioactive probe (5′ ABI4 or 5′ 4g16160) or the 3′ UTR of ABI4