Table 1.
Calibration data for all the compounds examined (n = 6)
| Compound | Linear dynamic rangea (μg mL−1) | Slope (a) | S a | Intercept (b) | S b | S xy | Correlation coefficient, r 2 | LOD (ng mL−1) | LOQ (ng mL−1) |
|---|---|---|---|---|---|---|---|---|---|
| Flavonoids | |||||||||
| (±)-CA | 0.5–10 | 10290 | 139 | −744 | 804 | 1194 | 0.9993 | 103.2 | 309.6 |
| (−)-EC | 0.5–10 | 11543 | 162 | 422 | 936 | 1389 | 0.9992 | 107.0 | 321.1 |
| RUT | 0.05–5 | 33445 | 290 | −700 | 703 | 1289 | 0.9997 | 15.4 | 46.2 |
| QUR | 0.05–5 | 34721 | 337 | − 1256 | 817 | 1497 | 0.9996 | 15.5 | 46.6 |
| HSD | 0.1–10 | 18112 | 230 | 3789 | 1230 | 2192 | 0.9992 | 32.0 | 96.1 |
| NHSD | 0.1–10 | 23060 | 247 | 1898 | 1319 | 2351 | 0.9994 | 31.5 | 94.4 |
| HST | 0.1–10 | 25611 | 280 | 2429 | 1494 | 2664 | 0.9994 | 32.1 | 96.3 |
| Isoflavones | |||||||||
| PUR | 0.04–3 | 68987 | 725 | 564 | 543 | 991 | 0.9996 | 10.4 | 31.2 |
| GLY | 0.02–1.5 | 74582 | 295 | 255 | 221 | 404 | 0.9999 | 3.9 | 11.7 |
| DA | 0.04–3 | 91377 | 1019 | 603 | 763 | 1393 | 0.9995 | 11.0 | 33.1 |
| GT | 0.07–3 | 65955 | 818 | −1549 | 1226 | 2212 | 0.9994 | 20.4 | 61.3 |
| BIO | 0.04–3 | 109929 | 784 | 1700 | 1041 | 2097 | 0.9997 | 12.5 | 37.5 |
aLinear regression analysis with the regression equation:
, in which x is the concentration in μg mL−1 and y is the peak area. S
a is the standard deviation of the slope, S
b the standard deviation of the intercept, and S
xy the residual standard deviation of the regression coefficient