. 2010 Oct 9;399(9):3211–3219. doi: 10.1007/s00216-010-4206-6

Table 2.

Intra-day and inter-day precision, accuracy, and absolute recovery of flavonoids and isoflavones from human urine (n = 6)

Compound	Added (μg mL⁻¹)	Intra-day				Inter-day
Compound	Added (μg mL⁻¹)	Measured (μg mL⁻¹)	C.V.^a (%)	Accuracy (%)	Absolute recovery (%)	Measured (μg mL⁻¹)	C.V.^a (%)	Accuracy (%)	Recovery (%)
Flavonoids
(±)-CA	0.80	0.66	0.78	0.83	81.85	0.66	0.64	0.57	82.34
	2.50	2.06	1.71	−1.11	82.43	2.04	1.51	−1.02	81.61
	8.00	6.73	1.80	−2.74	84.12	6.58	1.38	−2.16	82.27
(−)-EC	0.80	0.72	1.05	0.15	90.37	0.72	1.04	−0.57	89.83
	2.50	2.13	1.95	0.46	85.37	2.07	2.60	−3.01	82.81
	8.00	7.27	0.34	−0.15	90.94	7.15	0.85	−1.70	89.39
RUT	0.80	0.56	0.30	−1.07	70.35	0.56	1.94	−0.54	70.00
	2.50	1.69	0.47	−4.70	71.00	1.69	0.63	−4.57	67.73
	4.00	2.84	0.62	−3.21	70.95	2.87	0.63	1.04	71.66
QUR	0.80	0.62	1.88	−1.29	77.89	0.62	1.91	−0.12	77.81
	2.50	2.04	2.25	−2.08	81.56	2.06	2.21	0.97	82.29
	8.00	6.45	0.39	2.99	80.62	6.49	1.40	0.72	81.18
HSD	0.80	0.60	0.82	−1.73	75.41	0.59	2.54	−2.23	73.75
	2.50	1.92	0.49	−1.22	76.98	1.89	0.59	−1.59	75.75
	8.00	5.87	1.59	−0.54	73.35	5.77	1.26	−1.67	72.10
NHSD	0.80	0.64	2.99	−2.09	80.09	0.63	2.09	−0.96	79.29
	2.50	2.10	0.98	−0.12	84.11	2.09	0.98	−0.69	83.52
	8.00	6.60	2.27	−1.07	82.48	6.58	2.40	−0.17	82.31
HST	0.80	0.61	1.81	0.05	75.89	0.60	2.20	−0.34	75.66
	2.50	1.99	0.34	0.03	79.56	1.96	0.96	−1.63	78.29
	8.00	6.40	0.39	0.39	80.06	6.27	1.35	−2.06	78.38
Isoflavones
PUR	0.24	0.21	3.16	−0.90	86.19	0.20	3.15	−3.92	82.83
	0.75	0.62	1.96	−4.27	82.58	0.61	2.34	−1.55	81.26
	1.20	1.02	3.82	−2.47	85.27	1.01	2.77	−0.55	84.76
GLY	0.24	0.23	2.00	−1.27	95.18	0.22	2.89	−3.97	91.37
	0.75	0.61	1.80	−0.33	81.10	0.58	2.84	−4.94	77.13
	1.20	0.97	3.62	−0.64	81.07	0.93	3.68	−4.67	77.26
DA	0.24	0.23	3.41	−2.22	96.09	0.23	3.79	1.55	96.57
	0.75	0.67	0.77	−0.04	89.21	0.67	0.56	−0.44	88.85
	1.20	1.00	0.34	1.13	83.22	1.02	0.45	1.82	84.72
GT	0.48	0.41	1.17	−4.99	85.10	0.40	1.80	−2.34	83.14
	1.50	1.10	0.23	−4.09	73.40	1.04	0.56	−5.00	69.73
	2.40	1.75	2.94	−3.20	73.11	1.69	2.58	−3.83	70.33
BIO	0.24	0.22	2.10	−0.70	90.83	0.21	1.70	−2.26	88.74
	0.75	0.72	1.12	−4.90	96.58	0.70	1.31	−2.93	93.78
	2.40	2.12	2.09	−4.53	88.54	2.03	1.62	−4.37	84.64

^aCoefficient of variation of the concentrations measured