Fig. 3.

ORA59 and ERF1 bind to two GCC boxes in the PDF1.2 promoter in vitro. a After SDS–PAGE and Western blotting recombinant proteins were detected with anti-His HRP-conjugated antibodies. Positions of protein size markers are indicated in k Dalton. b EMSAs were performed with recombinant ORA59 and ERF1 proteins and radio-labeled SF, m1, m2 or dm fragments. ORA47 protein and wild-type or mutated AOC2 promoter fragments were used as control. The arrowheads mark the positions of protein-DNA complexes (C) and free probes (F)